From early development to full scale commercial supply, our fermentation experts partner closely with customers to define robust, scalable solutions for complex fermentation-based molecules. Supported by a globally integrated CDMO network and long-standing operational continuity, we bring what matters most: scientific depth, manufacturing reliability, and consistent quality.
Your Partner for
Fermentation Based APIs
Backed by decades of industrial fermentation experience and commercial scale infrastructure, TAPI is ready to support your fermentation development and manufacturing needs.
Smal molecules
Peptides
Enzymes
Fermentation at TAPI
A Legacy of Delivering Results
TAPI’s fermentation heritage spans decades of industrial manufacturing, supporting complex pharmaceutical programs from microbial process development through commercial production. Our experience covers a broad range of fermentation-derived APIs, including antibiotics, cholesterol-lowering compounds, immunosuppressants, and other specialized product categories like enzymes or peptides.
Our platform integrates classical and genetic strain development with automated high-throughput screening and continuous investment in advanced molecular biology capabilities. This foundation enables efficient development, reliable scale-up, and long-term supply under controlled pharmaceutical manufacturing conditions.
Capabilities
Microbs small in size, vast in potential
Built to support complex fermentation programs with the scientific depth, GMP-ready infrastructure, and process control needed to move from development to commercial supply.
- End to end fermentation CDMO offering strain development through commercial manufacturing.
- Advanced upstream process control and data driven optimization.
- Flexible downstream purification strategies across diverse molecule types.
- Integrated analytical development supporting process understanding and compliance.
Strain Development and Improvement
- Diverse set of strains – bacteria, filamentous bacteria, filamentous fungi, yeasts
- Design and creation of producer strain
- Existing strain improvement
- Classical – directed evolution with classical microbiological tools
- Molecular biology – targeted DNA manipulation with genetic tools
- HTS automated system
- Biolog: high throughput characterization
- Viaflo: electronic pipetting
- Hamilton: Automated Liquid Handling
- Qpix: Colony Picker robot
- Rapid Fire: high throughput MS based analytics
Fermentation Technology Development
- Lab scale development
- Lab scale fermentation in HTS wells and shake flasks (from 0.5mL to 500mL)
- PreSens: online Dissolved oxygen (DO) and pH monitoring during fermentation
- Liquid Injection System (LIS): feeding in shake flask
- Multiparameter Sensor (MPS): Online Biomass measurements in shake flask
- Scale up
- Industrial approach during development
- Well-equipped pilot scale fermentation (from 2L to 1000L)
- Downstream processing
- Wide range of equipment and techniques also for large volumes (e.g. preparative and ion exchange chromatography)
- Special demands (e.g. impurity isolation)
Recombinant Production Development
- Target molecules: Peptides, enzymes (In-house expression), small API molecules (e.g. secondary metabolites)
- Diverse hosts:
- Bacteria: E. coli (routine), Pseudomonas (emerging)
- Yeasts: Saccharomyces, Pichia
- Classical and advanced molecular biology techniques (e.g. CRISPR)
- Various IH cloning methods (RE/ligation; Gibson assembly, Golden Gate)
- Enzyme evolution in HTS (SSVL, Combinatorial, Random)
- Several transformation methods for a wide range of microbes
Biocatalysis
- End-to-end biocatalytic development platform
- From biocatalytic route scouting to GMP implementation – utilization of new tools such as RetroBioCat, Askos
- Probability scoring by reliable 5-tiered classification system
- Extensive analytical support in enzyme characterization
- Expanding expertise on enzyme classes – 22 different enzyme types expressed in-house
- Competitive in-house enzyme expression platform
- Excellent titers, 6-10 g/l achieved through E. coli expression platform
- Reliable kg-scale expression of KRED(2), TAm(3), IRED(2)
- Expression of enzymes and peptides in alternative hosts (eg. S. cerevisiae, P. pastoris, Pseudomonas sp.)
- State-of-the-art enzyme engineering
- Modern, automated, enzyme selection workflow: AI-assisted structure modeling and docking using AlphaFold, RFdiffusion, ProteinMPNN
- >5-fold activity increase during first cycles
- >2-fold increase in soluble expression achievable
Capacity
Scale Without Delay
TAPI operates two dedicated fermentation sites designed to support projects from early development through large scale commercial manufacturing. Modular infrastructure and harmonized process controls enable efficient scale up and smooth technology transfer across program stages.
- Integrated upstream, downstream, and analytical operations
- Development and GMP manufacturing capabilities across multiple scales
- Proven readiness for long-term commercial supply
- Regularly audited sites with a strong regulatory track record, including successful FDA inspections
Ready When You Are
“A multidisciplinary team with decades of fermentation experience, committed to unlocking the full potential of microbial technologies.”
Bioengineer & Fermentation Subject Matter Expert
22 years in TAPI
22 years in TAPI
Why TAPI For Fermentation
Built for Scale. Backed by Experience. Ready to Deliver.
Decades of industrial fermentation manufacturing experience, with large-scale bioreactors ranging from [X] to 115,000 L, supporting robust and reproducible commercial production.
Full GMP compliance and regulatory readiness for global submissions, supported by regularly audited sites and long-standing regulatory experience.
Proven track record across fermentation-based molecules, with more than [30+] products successfully commercialized, from kilogram to multi-metric ton scale.
Integrated expertise across microbiology, molecular biology, upstream and downstream processing, ensuring deep process understanding and end-to-end control.
Seamless progression from development to commercial production, enabled by structured technology transfer and harmonized processes across development, pilot, and GMP manufacturing.
Flexible infrastructure supporting multiple microbial species, including E. coli and other industrially relevant hosts.
